Publication Detail

The publication detail shows the title, authors (with indicators showing other profiled authors), information on the publishing organization, abstract and a link to the article in PubMed. This abstract is what is used to create the fingerprint of the publication within Collexis applications. If any grants are referenced by the publication, they will be listed here as well.

Residues in the membrane-spanning domain core modulate conformation and fusogenicity of the HIV-1 envelope glycoprotein.

Shang Liang; Hunter Eric (Profiled Author: Hunter, Eric)

Yerkes National Primate Research Center, Department of Pathology and Laboratory Medicine, and Emory Vaccine Center, Emory University, Atlanta, GA 30329, USA.
Virology 2010;404(2):158-67.

Abstract

The membrane-spanning domain (MSD) of human immunodeficiency virus type I (HIV-1) envelope glycoprotein (Env) is critical for its biological activity. Initial studies have defined an almost invariant "core" structure in the MSD and demonstrated that it is crucial for anchoring Env in the membrane and virus entry. We show here that amino acid substitutions in the MSD "core" do not influence specific virus-cell attachment, nor CD4 receptor and CXCR4 coreceptor recognition by Env. However, substitutions within the MSD "core" delayed the kinetics and reduced the efficiency of cell-cell fusion mediated by Env. Although we observed no evidence that membrane fusion mediated by the MSD core mutants was arrested at a hemifusion stage, impaired Env fusogenicity was correlated with minor conformational changes in the V2, C1, and C5 regions in gp120 and the immunodominant loop in gp41. These changes could delay initiation of the conformational changes required in the fusion process.

2 Originating Grant

• 1.

  Hunter, Eric

  STRUCTURE FUNCTION/ANALYSIS OF THE HIV ENV GENE PRODUCT

  1 September 1992 - 30 November 2007

  NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

  Total Funding: $ 1,773,288

• 2.

  Hunter, Eric

  STRUCTURE/FUNCTION ANALYSIS OF THE HIV ENV GENE PRODUCT

  1 September 1992 - 31 August 2002

  NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

  Total Funding: $ 2,630,052

Scientific Context

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